多酚杂志

本期目录

2024年, 第6卷, 第2期　刊出日期：2023-12-20 上一期    下一期

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#br# Mechanism of action of Balanophora involucrata polyphenolic compounds in the treatment of myocardial injury based on network pharmacology and molecular docking techniques Shipeng Duan, Ting Gao, Feifei Li, Xuehan Li, Haojun Shen, Fang Wang, Xiaoshu Zhang 2024 (2):  45-55.  摘要 ( 85 )   PDF(8713KB) ( 130 )   Abstract The objective of this work was to investigate the mechanism of action of Balanophora involucrata polyphenolic compounds in the treatment of myocardial injury. In the present study, Balanophora involucrata was extracted by refl uxing 75% of ethanol. The obtained extract was extracted with petroleum ether, ethyl acetate and n-butanol respectively. And the ethyl acetate layer was separated. The extract was prepared by silica gel column chromatography, sephadex LH-20 elution and thin layer chromatography. After that, the Swiss target prediction database was utilized to obtain the targets of Balanophora involucrata, and the Genecards, OMIM and TTD databases were used to predict and screen the targets of Balanophora involucrata for the treatment of myocardial injury. The active ingredient-target network was constructed using Cytoscape software, and the PPI network was mapped using String database and Cytoscape software. GO bioprocess enrichment analysis and KEGG pathway enrichment analysis were performed by Metascape software to predict the mechanism of action. Molecular docking was performed in Discovery Studio 2016 client software to verify the binding of Balanophora involucrata polyphenols to key targets. In this study, six polyphenolic compounds were isolated from Balanophora involucrata. By GO enrichment analysis, 1 614 biological processes (BP), 127 cellular compositions (CC), and 215 molecular functions (MF) were obtained; a total of 155 cross-targets were involved in the KEGG enrichment analysis. The PPI network showed that quercetin was the main active component of polyphenolic compounds against myocardial injury and that AKT1, EGFR, STAT3, SRC, ESR1, MMP9, HSP90AA1 and other related signals were associated with myocardial injury treatment. Finally, the multicomponent-multi-target-multi-pathway action of Balanophora involucrata was concluded, which provided new ideas and methods for further research on the mechanism of action of Balanophora involucrata in myocardial injury. 相关文章 | 计量指标

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Preparation, characterization and evaluation of kaempferol phospholipid complex: Improvement of its solubility and biological effect Dezhi Gao, Honghui Zhao, Fengmao Zou, Ming Yang, Jing Wang, Lina Fang, Xu Zhao 2024 (2):  56-64.  摘要 ( 76 )   PDF(1873KB) ( 30 )   Abstract Kaempferol (KA), as one of the fl avonoids, has extensive pharmacological properties. However, the poor solubility of KA severely limits its clinical application. In our study, the kaempferol phospholipid complex (KA-PC) has been prepared by solvent evaporation for the enhancement of the bioavailability of KA. KA-PC was verifi ed by scanning electron microscope characterization methods. Drug loading, solubility and long-term stability were measured. The characterization results showed that KA-PC was formed through the intermolecular interaction between KA and phospholipids. The solubility of KA-PC in water was 189 times higher than that of KA, and the solubility in n-octanol was also signifi cantly improved. Besides, pharmacodynamic studies showed that KA-PC can signifi cantly reduce the level of serum uric acid in mice without causing renal injury. This study expanded the clinical application of KA by preparing KA-PC. 相关文章 | 计量指标

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Mechanism of Rosae Rugosae Flos flavonoids in the treatment of hyperlipidemia and optimization of extraction process based on network pharmacology Yunxiao Xia, Aijinxiu Ma, Zihan Hou, Xu Zhao 2024 (2):  65-77.  摘要 ( 101 )   PDF(2865KB) ( 50 )   Abstract This study aims to identify a natural plant chemical with hypolipidemic effects that can be used to treat high cholesterol without adverse reactions. Through network pharmacology screening, it was found that Rosae Rugosae Flos (RF) flavonoids had potential therapeutic effects on hyperlipidemia and its mechanism of action was discussed. TCMSP and GeneCards databases were used to obtain active ingredients and disease targets. Venn diagrams were drawn to illustrate the findings. The interaction network diagram was created using Cytoscape 3.8.0 software. The PPI protein network was constructed using String. GO and KEGG enrichment analysis was performed using Metascape. The results revealed 2 active flavonoid ingredients and 60 potential targets in RF. The key targets, including CCL2, PPARG, and PPARA, were found to play a role in multiple pathways such as the AGE-RAGE signaling pathway, lipid and atherosclerosis, and cancer pathway in diabetic complications. The solvent extraction method was optimized for effi cient fl avonoid extraction based on network pharmacology prediction results. This was achieved through a single factor and orthogonal test, resulting in an optimum process with a refl ux time of 1.5 h, a solid-liquid ratio of 1:13 g/mL, and an ethanol concentration of 50%. 相关文章 | 计量指标

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Qualitative and quantitative analysis of characteristic free and bound phenolics in three colored quinoas Jianxin Song, Dezhi Gao, Xiaodong Wang 2024 (2):  78-86.  摘要 ( 96 )   PDF(2826KB) ( 95 )   Abstract Quinoa is a good source of phenolics, which both exist as free and bound forms. In order to mark clear the characteristic free and bound phenolics in different quinoa samples, in this study, characteristic free and bound phenolics in three colored quinoas including WQ (white quinoa), RQ (red quinoa) and BQ (black quinoa) were investigated. Result showed a total of 14 phenolics both acted as free and bound form were analyzed in three colored quinoas (WQ, RQ and BQ). Gallic acid, vanillic acid, epicatechin, p-coumaric acid and quercetin existed both as free and bound forms were common phenolics in quinoas. The highest total free phenolics (238.10 mg/kg) and bound phenolics (3 377.75 mg/kg) were presented in WQ and RQ, respectively. It indicated WQ and RQ were respectively good source of free and bound phenolics. Moreover, characteristic free and bound phenolics in three colored quinoas could be well analyzed by principal component analysis ( PCA), indicating it was an eff ective and reliable method in distinguishing three colored quinoas based on their characteristic free and bound phenolics, respectively. 相关文章 | 计量指标